diff --git a/isort/output.py b/isort/output.py
index 6b3401089..ee7406ca8 100644
--- a/isort/output.py
+++ b/isort/output.py
@@ -54,12 +54,15 @@ def sorted_imports(
                 key=lambda key: sorting.module_key(
                     key, config, section_name=section, straight_import=True
                 ),
+                reverse=config.reverse_sort,
             )
 
         from_modules = parsed.imports[section]["from"]
         if not config.only_sections:
             from_modules = sorting.naturally(
-                from_modules, key=lambda key: sorting.module_key(key, config, section_name=section)
+                from_modules,
+                key=lambda key: sorting.module_key(key, config, section_name=section),
+                reverse=config.reverse_sort,
             )
 
         straight_imports = _with_straight_imports(
@@ -233,6 +236,7 @@ def _with_from_imports(
                     config.force_alphabetical_sort_within_sections,
                     section_name=section,
                 ),
+                reverse=config.reverse_sort,
             )
         if remove_imports:
             from_imports = [
diff --git a/isort/sorting.py b/isort/sorting.py
index 2cfe60bf5..e8c355bab 100644
--- a/isort/sorting.py
+++ b/isort/sorting.py
@@ -96,7 +96,9 @@ def section_key(line: str, config: Config) -> str:
     return f"{section}{len(line) if config.length_sort else ''}{line}"
 
 
-def naturally(to_sort: Iterable[str], key: Optional[Callable[[str], Any]] = None) -> List[str]:
+def naturally(
+    to_sort: Iterable[str], key: Optional[Callable[[str], Any]] = None, reverse: bool = False
+) -> List[str]:
     """Returns a naturally sorted list"""
     if key is None:
         key_callback = _natural_keys
@@ -105,7 +107,7 @@ def naturally(to_sort: Iterable[str], key: Optional[Callable[[str], Any]] = None
         def key_callback(text: str) -> List[Any]:
             return _natural_keys(key(text))  # type: ignore
 
-    return sorted(to_sort, key=key_callback)
+    return sorted(to_sort, key=key_callback, reverse=reverse)
 
 
 def _atoi(text: str) -> Any: