diff --git a/leviosam-test.py b/leviosam-test.py index e3f3079..3fa6384 100644 --- a/leviosam-test.py +++ b/leviosam-test.py @@ -1,3 +1,4 @@ +import pysam import unittest import subprocess @@ -143,5 +144,57 @@ def test_picard_validate(self): print('[PASSED] Picard AddOrReplaceReadGroups & ValidateSamFile') +class Chain(unittest.TestCase): + def compare_aln(self, gold, result): + if gold.is_unmapped or result.is_unmapped: + return + self.assertEqual(gold.reference_start, result.reference_start) + + def read_paired_end(self, fn): + f = pysam.AlignmentFile(fn) + reads1 = {} + reads2 = {} + for r in f: + if r.is_read1: + reads1[r.query_name] = r + elif r.is_read2: + reads2[r.query_name] = r + else: + print(f'Invalid record in {fn}. Please check') + print(r) + exit(1) + return reads1, reads2 + + def test_paired_endh38_to_h37(self): + INPUT_FN = 'testdata/bt2-paired_end-grch38.sam' + GOLD_FN = 'testdata/bt2-paired_end-grch37.sam' + OUTPUT_PREFIX = 'testdata/bt2-paired_end-grch38_to_grch37' + OUTPUT_FN = OUTPUT_PREFIX + '.sam' + process = subprocess.Popen( + ['./leviosam', + 'lift', '-C', 'testdata/hg38ToHg19.over.clft', '-a', INPUT_FN, + '-p', OUTPUT_PREFIX], + stdout=subprocess.PIPE, stderr=subprocess.PIPE) + stdout, stderr = process.communicate() + print(f'Lifted {INPUT_FN}') + + reads_gold1, reads_gold2 = self.read_paired_end(GOLD_FN) + reads_lift1, reads_lift2 = self.read_paired_end(OUTPUT_FN) + + for _, (name, v) in enumerate(reads_gold1.items()): + result = reads_lift1.get(name) + self.assertFalse(result is None) + self.compare_aln(v, result) + for _, (name, v) in enumerate(reads_gold2.items()): + result = reads_lift2.get(name) + self.assertFalse(result is None) + self.compare_aln(v, result) + + cmd = f'rm {OUTPUT_FN}' + process_rm = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + stdout, stderr = process_rm.communicate() + print(f'Cleaned up {OUTPUT_FN}') + + if __name__ == '__main__': unittest.main() diff --git a/testdata/bt2-paired_end-grch37.sam b/testdata/bt2-paired_end-grch37.sam new file mode 100644 index 0000000..1117941 --- /dev/null +++ b/testdata/bt2-paired_end-grch37.sam @@ -0,0 +1,233 @@ +@HD VN:1.0 SO:unsorted +@SQ SN:chr1 LN:249250621 +@SQ SN:chr2 LN:243199373 +@SQ SN:chr3 LN:198022430 +@SQ SN:chr4 LN:191154276 +@SQ SN:chr5 LN:180915260 +@SQ SN:chr6 LN:171115067 +@SQ SN:chr7 LN:159138663 +@SQ SN:chrX LN:155270560 +@SQ SN:chr8 LN:146364022 +@SQ SN:chr9 LN:141213431 +@SQ SN:chr10 LN:135534747 +@SQ SN:chr11 LN:135006516 +@SQ SN:chr12 LN:133851895 +@SQ SN:chr13 LN:115169878 +@SQ SN:chr14 LN:107349540 +@SQ SN:chr15 LN:102531392 +@SQ SN:chr16 LN:90354753 +@SQ SN:chr17 LN:81195210 +@SQ SN:chr18 LN:78077248 +@SQ SN:chr20 LN:63025520 +@SQ SN:chrY LN:59373566 +@SQ SN:chr19 LN:59128983 +@SQ SN:chr22 LN:51304566 +@SQ SN:chr21 LN:48129895 +@SQ SN:chr6_ssto_hap7 LN:4928567 +@SQ SN:chr6_mcf_hap5 LN:4833398 +@SQ SN:chr6_cox_hap2 LN:4795371 +@SQ SN:chr6_mann_hap4 LN:4683263 +@SQ SN:chr6_apd_hap1 LN:4622290 +@SQ SN:chr6_qbl_hap6 LN:4611984 +@SQ SN:chr6_dbb_hap3 LN:4610396 +@SQ SN:chr17_ctg5_hap1 LN:1680828 +@SQ SN:chr4_ctg9_hap1 LN:590426 +@SQ SN:chr1_gl000192_random LN:547496 +@SQ SN:chrUn_gl000225 LN:211173 +@SQ SN:chr4_gl000194_random LN:191469 +@SQ SN:chr4_gl000193_random LN:189789 +@SQ SN:chr9_gl000200_random LN:187035 +@SQ SN:chrUn_gl000222 LN:186861 +@SQ SN:chrUn_gl000212 LN:186858 +@SQ SN:chr7_gl000195_random LN:182896 +@SQ SN:chrUn_gl000223 LN:180455 +@SQ SN:chrUn_gl000224 LN:179693 +@SQ SN:chrUn_gl000219 LN:179198 +@SQ SN:chr17_gl000205_random LN:174588 +@SQ SN:chrUn_gl000215 LN:172545 +@SQ SN:chrUn_gl000216 LN:172294 +@SQ SN:chrUn_gl000217 LN:172149 +@SQ SN:chr9_gl000199_random LN:169874 +@SQ SN:chrUn_gl000211 LN:166566 +@SQ SN:chrUn_gl000213 LN:164239 +@SQ SN:chrUn_gl000220 LN:161802 +@SQ SN:chrUn_gl000218 LN:161147 +@SQ SN:chr19_gl000209_random LN:159169 +@SQ SN:chrUn_gl000221 LN:155397 +@SQ SN:chrUn_gl000214 LN:137718 +@SQ SN:chrUn_gl000228 LN:129120 +@SQ SN:chrUn_gl000227 LN:128374 +@SQ SN:chr1_gl000191_random LN:106433 +@SQ SN:chr19_gl000208_random LN:92689 +@SQ SN:chr9_gl000198_random LN:90085 +@SQ SN:chr17_gl000204_random LN:81310 +@SQ SN:chrUn_gl000233 LN:45941 +@SQ SN:chrUn_gl000237 LN:45867 +@SQ SN:chrUn_gl000230 LN:43691 +@SQ SN:chrUn_gl000242 LN:43523 +@SQ SN:chrUn_gl000243 LN:43341 +@SQ SN:chrUn_gl000241 LN:42152 +@SQ SN:chrUn_gl000236 LN:41934 +@SQ SN:chrUn_gl000240 LN:41933 +@SQ SN:chr17_gl000206_random LN:41001 +@SQ SN:chrUn_gl000232 LN:40652 +@SQ SN:chrUn_gl000234 LN:40531 +@SQ SN:chr11_gl000202_random LN:40103 +@SQ SN:chrUn_gl000238 LN:39939 +@SQ SN:chrUn_gl000244 LN:39929 +@SQ SN:chrUn_gl000248 LN:39786 +@SQ SN:chr8_gl000196_random LN:38914 +@SQ SN:chrUn_gl000249 LN:38502 +@SQ SN:chrUn_gl000246 LN:38154 +@SQ SN:chr17_gl000203_random LN:37498 +@SQ SN:chr8_gl000197_random LN:37175 +@SQ SN:chrUn_gl000245 LN:36651 +@SQ SN:chrUn_gl000247 LN:36422 +@SQ SN:chr9_gl000201_random LN:36148 +@SQ SN:chrUn_gl000235 LN:34474 +@SQ SN:chrUn_gl000239 LN:33824 +@SQ SN:chr21_gl000210_random LN:27682 +@SQ SN:chrUn_gl000231 LN:27386 +@SQ SN:chrUn_gl000229 LN:19913 +@SQ SN:chrM LN:16571 +@SQ SN:chrUn_gl000226 LN:15008 +@SQ SN:chr18_gl000207_random LN:4262 +@PG ID:bowtie2 PN:bowtie2 VN:2.3.5.1 CL:"/home/cnaechy1/miniconda3/bin/bowtie2-align-s --wrapper basic-0 -x /home/cnaechy1/data_blangme2/fasta/grch37/bt2/hg19 -S bt2-paired_end-grch37.sam -1 raw_reads/paired_end_1.fq -2 raw_reads/paired_end_2.fq" +A00217:76:HFLT3DSXX:1:1101:1036:12743 81 chr11 63961827 42 150M = 63961459 -518 TCTTATTATTAATGTGATTAATTTTGAGTCTTCACCCCTGAGAAATGTCAGTCTGGTAGGGTATGGGACACAGTAATTCTAGTGAACTCATTTCATAGTGATGTGCTTTCCTTTATTTGGACAGAGAGAAAATTCATGAACCCTTTCAAC ???????????????????????????????????????????????????????????????????????????????????????????????????????????+?????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-4 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1036:12743 161 chr11 63961459 42 150M = 63961827 518 GNCGGATGTGCCTCTGCTATTTGGATGGATCCTTACAGTAGCAGCCTTGGCCTCGTAGCCTACTCCTCTCCTTTTTTTCTCTTTGGCCTTCATGTGAATACTCATTTTTCCCCCATAAAAGTAGAATTGTTCTTGTTTGAGTTCGTCTTC ?#?5?????????????????????????????????????????????????????????????????????????????5????????????????????????????????+?+?????5???????????+?+?????+??????? AS:i:-4 XN:i:0 XM:i:2 XO:i:0 XG:i:0 NM:i:2 MD:Z:1G132T15 YS:i:0 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1624:2832 83 chr4 32144676 42 150M = 32144394 -432 TTACACTCCCACCAACAGTGTAAAAGCGTTCCTATTTCTCCACATCCTCTCCAGCATCTGTTGTTTCCTGACATTATAATGATCACTATTCTAACTGGCATGAGATGGTATGTCATTGTGGATTTGATTTGCGTTTCTCTAATGCCCAGT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XS:i:-40 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-8 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1624:2832 163 chr4 32144394 42 27M1D123M = 32144676 432 TGTTCTTGTGTTAGTTTGCTGAGAATGTGGTTTCCAGCCTCATCCAGGTCCCTGCAAAGGACATGAACTCATCCATTTTTATGGCTGCATAGTATTCCATGGTGTATATGGGTTGCTTCCAAAGCTTTGTTGTTGTGAACAGTGCCATAA ??????????????????????????????????+???5??????????????????????5???????????????????????????????5???+???????????????????????????????????????????????????? AS:i:-8 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:27^A123 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1931:33019 99 chr13 43750671 42 5M1D145M = 43750919 398 TACTCTATGTATTTTGGTGCTCTGTTATTAGGTGCATAGGTGCAAAAATTTTGTTTTGTATTGCATTGAATTGATACATCAATTTAGATAAATTGTCATCTTGGCAATATTGACTCAATATGTGTATATAAGGGTACTTCAAAACATTTG ????????????????????????5????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-8 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:5^T145 YS:i:-21 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1931:33019 147 chr13 43750919 42 150M = 43750671 -398 TTTAGCTCATCCATAAAGAATTGTGGGTATTGGGATTTTAATCATGTAAATGCAGTCATTTTTTTTAATATTAACTGAAGAATGGTAGGTGCCCTTTAAAGATATTTTAATATAAGAAAAAAAAAAAAACCAGAAAAATGTCAGAAGGAG ??5????+??5?+????++????+?55?+??5+??+???????+???+?+?5?5??++??????+?+???????+?????++????5??????+??????+????????++??????????????????????????????????????? AS:i:-21 XN:i:0 XM:i:7 XO:i:0 XG:i:0 NM:i:7 MD:Z:12C10A4C6A11C16G45G39 YS:i:-8 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:9220 99 chr17 60701648 42 150M = 60701934 436 CCTGAGGTCAGGAGTTTGAGACCAGCCTGGCTAACATGGTGAAACCCCTCTCTACTAATTAGCTGGGCATGGTGGCACGTGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGGACCTAGGAGGTGGAGGTTGCAG ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:9220 147 chr17 60701934 42 150M = 60701648 -436 AGGTTGTACAGGAAGCATGATGCTGGCATCTGCTCAGCTTCTAGGGAGGCCTCAGGAAACTTAAAATCATGGCGGAAGGGATGCAGCAGTTCACATGGCTGGAGCAGGAGCGAGACAGAGAGTGGAGAGGTGCTGCACACTTTTAAATGA ????5??????????????????55????????????????????????????????????5?+????+????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1470:1532 99 chr1 188168154 42 105M4I41M = 188168436 432 ATATTTACATGGTTTCAAAATTCCTTCCCACCAATTAACTATTAATTACAAAATGAAGGGTTTCTGCTTCTGTAACTCCTGTCGATCCAATTCGTTTATAACTACAAGAAAGAAAGAAAGAAAGAGAGAGAGAGAGAGGACGAGAAGAGA ????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????+????????? AS:i:-20 XN:i:0 XM:i:1 XO:i:1 XG:i:4 NM:i:5 MD:Z:136A9 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1470:1532 147 chr1 188168436 42 150M = 188168154 -432 TGCAGATGTGAGACTGCCAATTTTAGGAGAATAAAACTTAAAAAAAAATTATCAGTCTTACTGGCTTAAAGAAAAAGTTGGAGCCACCACATCTGGAGAGTGAAGAAGTTCCACATTAAGGGAGAAACAGTGCCAAATTATGTGAATAAA ????????????????5????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-20 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1027:7059 99 chr12 65879710 42 150M = 65880039 479 TGAGTTAATATATGTATAAATATTAGGACTATTGGTATATTTAAATTCATTTAAACTATCTTACTTTGTGCTTTATTTTTTTCTGCTTTTGCTATGCTTTTGGAAAAATCGATTTTCTTTGCTTTTTCACTTATTGTGTCCTACTAGTTA ????????????5???????????????????????????????????????????????????????????????5??+?????????????????????????????????????????????????????+????+?+?????5??? AS:i:-4 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:76A73 YS:i:-13 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1027:7059 147 chr12 65880039 42 150M = 65879710 -479 TACCACTCACAAAATAAACACTATTATACTAACTACACATAAAGATAATGACAACTTGACTACATGTTTGCTAATTTCTTTGCTCACAATTCTCTCCTGTTTCTTCAGTCTTTCTTTTGAGATCATTTTCTTACTNCTGAAGTTCATTNA ?+?+?+??++??????????????????+???+????+?????????????+??????+??????????5????????????+???+??????????????????+?5?+?????????????????????????#?+??????????#? AS:i:-13 XN:i:0 XM:i:5 XO:i:0 XG:i:0 NM:i:5 MD:Z:1C2C53C76C12C1 YS:i:-4 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1118:10410 83 chr10 99489775 42 150M = 99489523 -402 CTGGGATTACAGGCATGAGCCACCATGCCCAGCCCCCACTAACTTCTTATTTTCCATGTTATTTATTCATTTTTAAACAAGCATTCATTGAGTACCTACTATGCCACAGCTCTATACTTGGTAGAAACTGAGGCTGCCAAAATGTTAAAT ???????+???????????????????????????????????????????????+?????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-8 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1118:10410 163 chr10 99489523 42 9M1I140M = 99489775 402 CAACTTCTATTTTTTTTTTTAAGACGGAGTCTCACTCTGTCGTCCGGGCTGGAGTGCAGTGGCACTGCAACCTTCTGCCTCCCAGGTTCAAGCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCGCCACCATGACC ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????+???????5???5?5????????? AS:i:-8 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:149 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1271:15749 77 * 0 0 * * 0 0 TTATGTACAGTATATAATAGATATTTGGGGTACTTTTATATTTTATGTACAGTATACAATATATATTTTGGCTACTTTGATATTTTATGTACAGTTTACAATATATATTTTGGGTACTTTGATATTTTACGGACAGTATAAAATACACAG ???????????????????????????????????????????????????????????????????????????????????????????????????????????++????????????????????????????????????????? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1271:15749 141 * 0 0 * * 0 0 ATTTATAATAAACTGTACATAAAATATCAATGTACTCAAACTATATATTATGTGTGGTACATAAGATATCAAAGTACCCATACTATATAGTATATACTGTACTTAAAATATCAAAATACCCAAAATATATATTATATACGTTACATAAAC ????????????????????????????????????????????????????????????????+???????????????????????????????5????????????????????????????????????????????????????? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1443:13041 83 chr8 25257948 40 66M9I75M = 25257634 -455 AGGTTGCAGTGAGCCGAGAACACGCCACAGCACACCAGCCTGGGCAACAGAGTGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAGAAAAGTTCATCTGTTGGTTTCCTGGCATGTGTTTCCAGCTGAGAGATGCTGGCCTCATTT 5?+?+???5++5??????++?+????+?55?+?+??5???+????????+?5+??+??+???+++??????+????????????5????????5?????????????+?????????????????????????????????????????? AS:i:-38 XN:i:0 XM:i:2 XO:i:1 XG:i:9 NM:i:11 MD:Z:19T13T107 YS:i:-10 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1443:13041 163 chr8 25257634 40 150M = 25257948 455 AAGCACCACCTATCTATGTATTTGTTTGCTCGGCCGGCAGTTAAACTGCCCAGGGTTGACGGAGCCTTCTTAAAGCTGAGATCAAAGTTCATCGGTTGGGGCCAGGCCCAGTGGCTTATGCCTGTAATCCCAGCACTTTGGGAGGCTGAA ??????????????????????????????????????????????5????????+?????5?????????????????????????????????????????????????????5?????????????????????++?5?????5??+ AS:i:-10 XN:i:0 XM:i:2 XO:i:0 XG:i:0 NM:i:2 MD:Z:95C7G46 YS:i:-38 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:1924 97 chr12 98716376 42 150M = 98716755 528 TTTAATCTATTACAATAAACTAAATCTTTTTTGTTTTTTTTTTTGATACGTTATACTTGTACATATTTAGGGGTACATGAGAAATTTTGTTACATGCATGAATGTGTAATGGTCAAGTCAGTGTATTTAGTATGTATCATTTCTATGTGT ????????????????????????????5????????????????????????????????????????????????????????????????????????????????????????????????????+???????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:5G144 YS:i:-28 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1063:1924 145 chr12 98716755 42 145M1I4M = 98716376 -528 TAACTCTCACATATGAGTGAGAACATGTGGTATTTGTCTTGCCTGGTTTATTTCACTTAACCTAACGGCCTCCAGTTCCATCCAGTCATCCATTTATTGCTGCAATTGACAGGATTTCATTCTTTTTCATGGCTGAATAATATTTCATTG ?????????????????????????????????????????????????????????5???????????????????5??????????????????????????????????????????????????+????????????????????? AS:i:-28 XN:i:0 XM:i:4 XO:i:1 XG:i:1 NM:i:5 MD:Z:116C28C0A1T0 YS:i:-5 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1344:26960 77 * 0 0 * * 0 0 TTCGAATCCATTCCATTCCAGTCCATTCCATTCGATTCCATTCCATTCATTTCAATTCCATTCCATTCCATTCCATTACATTCCACTTGATTCCTCTCCATTCCCTTCCACTGCATTCCGTTCGATTCCATTCCATTGCATTGCATTCCA ???5????????????????????????????????????????????????????????????????????????????+????????????????????????????????????????????????????????????????????? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1344:26960 141 * 0 0 * * 0 0 AAATAGAATGGACTCAAAAGAAATGGTCTTGAATGGAACTTATTCGAATATAATCTAATCGAATGGAATGCAATAGTATGGAATGGAATCGAATGGAATGGAATCGAATGGATTGGAACGAAATCGAATGGAACGGAAGAGAATAGACTC ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1027:4711 81 chr11 128427280 42 150M = 128426848 -582 TTGAATGAAATAATGTATGAGAAAATTGTATATAAAGCAAATTAAAATATAAAATTGCATTTAATAAAACTATATATTGTATGAAATGCATGTGATAAGTATGTGAGGCCTGGTCTTCAATATAAGTTAGGCATTATTTTAATTTAAGAG ??????????????????????????????????+5?????????????????+???????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-2 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1027:4711 161 chr11 128426848 42 150M = 128427280 582 ANATTGTCCATTTGNCCCAATTTTCGAAGACTTGTGCCACACTCTTAGTAATTTTATGAACTCCATAAAATATACAATCCCAATCTACATAGTACTCTCTAAGGAAGACAGATGTGCTGTGTGGAAGGTCATGTTTTGCTGTGTTTAAAA ?#????????????#?????????????????????????????????????????????????????????????????????????5?+??????????????????+?????????+???+?????????????????????????? AS:i:-2 XN:i:0 XM:i:2 XO:i:0 XG:i:0 NM:i:2 MD:Z:1A12C135 YS:i:0 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1054:20071 77 * 0 0 * * 0 0 TTCTGAGAATGAGTCTGTCTAGTTCTTATATGAAGATATTTGGTTTTCCACCATCGGCTTCAAATCACCCCAAATATGCACTTGGAAATTCAACAAAAAGAGTGTTTCAAAACTGCTCTATCAAAAGGAGGGTTTAACTCTGTCAGCTGA ?????????????????????????????????????????????????????????????????????????+?????????????????????????????????????????????+?????????????????????????????? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1054:20071 141 * 0 0 * * 0 0 ATCTTCACATAAAACCTAGACAGATTCATTCTCAGAAACTACATTGTGATGTGTGCATTCAACTCACAGAGTGTAACGTTTCTGTTGATAGAGCAGATTTGAAACACTCTGTAAAGTCTGCAAGGGGATAATTGGACCTCTCTGAGGCAT ????????????????????????????????????????5??++?+?????????????????????????+??????+??5+????+????????????????????5??5??????????????+?+???????????+???????+ YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1145:26835 83 chr11 44364039 42 133M1D17M = 44363792 -398 AGGCATGAGCCGTCATGCCTGGTCTTGATTCTGCTTTTAAAACAAACAAAAAATACTCACAGAAGATAATCCAGAATCATGTGTATCTAAGTAGTAACAGGGATTATCTCTAAAAAGTGGGATCTGGGGTGGATTTTTTTTACTTCTTGT ?????????????????????????????????????????????????????+???????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-13 XN:i:0 XM:i:1 XO:i:1 XG:i:1 NM:i:2 MD:Z:59T73^T17 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1145:26835 163 chr11 44363792 42 150M = 44364039 398 TTATTTAGAGTCTGCATCTTGCCCTGTTGCCCAGGCTGGAGTGTACTGGCGTGATCATGGCTCACTGCAGCTTTGACCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCCTCCCAAGTAGCTGGGGCCAAAGGCTGGCTAACTCTGTTAA 5?????????????????????????????????????????????????????????????????????????????????????????????????????????????????5????????????????????????5?????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-13 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:7310 83 chr13 26652231 21 150M = 26652056 -325 CCCTGACTTAACTGGCTTAAATAATAAGACAACTTATTATTAAGGGTGGCCCAATTGCTGATTGAGACAGCCGCTCAAATATATCACTGCAGGGACCCAGTTTTCCCCACCGCTCTGTTTCACCATTCTGAGCGCTGGCCTTGCCTCCAC ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????5?????????????? AS:i:0 XS:i:-55 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-4 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:7310 163 chr13 26652056 21 150M = 26652231 325 TTCTGGTTGTATGTTTGCTTTTTGCATGCTCTAATGTTTAATGTTTTTGAGGGCAAAAGTTTTAAAGGTTTGAAAATGATAAATGCTAGCATTTCAAATATGGTAATCTGGTACTATTTTTAAAGTAATTTATGTTAGGTAAATTGGGAT ?????5?????????????????????????????????????????????????????????5????????????????5???????????????????????+???????55?5????????????????????????+????????? AS:i:-4 XS:i:-24 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:5T144 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1371:29199 81 chr9 8011138 42 150M = 8010779 -509 TCTCATCAAATAATCAAGAAATATCAACCACCAGAGAAAGCGAGAGACTGGGGGTCCTCACCATGAAGAAATTTCATTAACTCTTCTGAGGGCACCTCTGAGAGATTACCTGGGGGACTTTATCTGCGTAATAAGCCAAGAGAATCATTT ?????????+??+?????????????????????????????????????????????????+????????????????????+?????????????????????????????????????????????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:125A24 YS:i:-16 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1371:29199 161 chr9 8010779 42 73M1D77M = 8011138 509 CTGGGAACAAATAGATGACTGGCACATACACGTATGTGTTTATTTCCCTTAGGTAAATTAATACATTGCTTACAAAAAAAAACAGAAGTGTAGAATTATATGACCTCAGATATAGAATATTGTTGGGGTACAGAAAATGATTCCCCCAAA ?????????????????????????????5?????????????????????????+??????????????+???????????????++???????????????????????5?5???+????????????????+????????+???5?? AS:i:-16 XN:i:0 XM:i:2 XO:i:1 XG:i:1 NM:i:3 MD:Z:26C46^A14T62 YS:i:-5 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1045:14763 83 chr10 114467706 42 150M = 114467470 -386 AGTCCTGGTTGCACTTTATTAAACATCTAGCCAATTTTTTCACCCCTGTTCCTGGTGAAGTTTATTTAGGTAAAAGGTTGATAACATTCTTTACCGCCAAAGAAAGCAGGTTCATTTCTGTTAGACAGTTCTATCCTGATAACATTGAAG ????????????????????????????????????????????????+?????????????????????????????+????????????????????????????????????+?????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:14763 163 chr10 114467470 42 150M = 114467706 386 TTGGCATTTAAGTTTTCCAGACCTGTCTCTGATTTGCTCTTACTTTGCATAAAAATTTTAAAGTTATTGTCCTAAATTGGAAGATGATGTATTTTAACATAAAGAGTATTAGACTCTGCCAGAATTATTTATTTGAGATTGACTATGAGC ???????????????????????????????????????????5????5??????????5?????5????????????????????????++???5???????????????????????????++???+?+?????????????+????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1118:19680 99 chr1 199841600 42 150M = 199841796 345 TAGTAATGTTGAAAATGTATATGTCCTTTACCTTTACAATTTCAGTCATAGTTATATGCCCTGTCCCATACCATACTATAAGAATGTTCATAAGAGCATTATTAGTAACAGCCTGTAATAGATGAAAAATGAAAAAATTACACATAGTGT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-8 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1118:19680 147 chr1 199841796 42 109M1I40M = 199841600 -345 GGGATCACTAAAGAGGAATGTGTTTGGGGAGGGGCATACAGGAGACTTTTAAGATACTGGCATTGTTCTTTTTCTCCATTTGGATAGCAATTACAGAAGTGTTCCTTAATTTTTTTCTTTTTTTTAGACAAAGTCTTGCTCTGTCACCTA ????????5????????????????????????????????????????+????5???????+???????????+?????????????????????????????????????????????????5????????????????????????? AS:i:-8 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:149 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:9455 97 chr20 37402373 42 150M = 37402778 554 TAAAACCACACTCAATACCCATCAACTCCCTTCCCCCTGCCTCTGTCCATCCCCTACTCTTTGCAACCACCATTCTACTTTGTAAGAGTTTTTCTTTAGATAACTCACATAAGAGAAGCCATACAGTATTTGTTGTTGTGTAACTGGCCT ?????????????????????????????????????????????????????????????????+??+???+??????????????????+???+???????????????????????????????+????+????+???????????5 AS:i:-8 XN:i:0 XM:i:2 XO:i:0 XG:i:0 NM:i:2 MD:Z:55G81T12 YS:i:-19 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1054:9455 145 chr20 37402778 42 135M1I14M = 37402373 -554 ATCATATGGGAGTTCTATTTTTAATTTTTTGTGGAACACTCGTACTGTTTTCCATAGCAGCTGCACCATTTTCAGTCCCACCATCAGTGCATGAGAGTTCCAGTTCCTCCACATCTTCACCACCTCATTATGCTGTTTTTTTTTTTTTTG +?????????5?????+??????+?????5?5????????5????????+????????5??5???????????5++????+?????????????????????????????????????????+??????????????????????????? AS:i:-19 XN:i:0 XM:i:3 XO:i:1 XG:i:1 NM:i:4 MD:Z:9T64T47A26 YS:i:-8 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1723:7639 97 chr5 117479053 40 150M = 117479589 685 CAAAATGTGTGTTCATTTAAAAATTTTCATGTAGAAGACGATTTGCTCAAGATAGTTCCAGAATATCCTTGAATAGCAAAAAGGTGAGAGCAAGAAGTGGAAAGGGCAATGGCAATGTAGGGAGAGTTAAAGTCGAATTTAGAAGTGAGA ???????5?????????????????????????????????????????????????????????????????????????????????????????????????????????????+???????+???????????+???????????? AS:i:-3 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:125T24 YS:i:-48 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1723:7639 145 chr5 117479589 40 35M3I44M2D68M = 117479053 -685 TGTGATAAATCCTAAATCATTAACTGGGACTAGAAGGGGGTCCTGGATCTTCTAGCCCCTGGACTAAGCCTATTTACATGTGTATATATATGGTTATATATATAGGTCTATGGAGATATATATATATATATATATACACACACAAACACA ??+????+???????+??+???????????5??????+???????????????5???5?????????????????????????????????5?+5+???????????+5???+?????????????????????+????5?5??55?5?? AS:i:-48 XN:i:0 XM:i:6 XO:i:2 XG:i:5 NM:i:11 MD:Z:1A0G15C60^TA32T19C9T5 YS:i:-3 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1072:21010 83 chr1 218144875 42 150M = 218144585 -440 TTTTTGTATGGGTTTTTGTGTCTCAATTTCCTTCAGTTCAGCTCTGATTTTGGTTATTTCTTGTCTTCGGCTGACTTTGAGGTTGGTTTGCTCTTATTTTTCTAGTTTCTGTAGGTGTGATGTTAAGCTGTTAATTTGAGATCTTCCTAA ????+??????????????????????+?????????????????????????+?????????????????????????????????????????????????????????????????????????????????????????5?????? AS:i:-15 XN:i:0 XM:i:3 XO:i:0 XG:i:0 NM:i:3 MD:Z:10C99C23A15 YS:i:-11 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1072:21010 163 chr1 218144585 42 124M2D26M = 218144875 440 CTCATTATTCGTCTGTTCAGGGAATCAATTTCTTCCTGGCTCAATGTTGGGAGGTTGTGTTTCCAAGAATTTATGCATTTCTTCTAAGTTTTCTAGTTTGTGTGTACAGAATTGTTCTTAGTAGTCTGATCATTTTTTCTGTGTTTCTGT ?????????????????????????????????????????????????????????????????????????????????????5???????????+??????????++???????????????????+???????+?5+????????? AS:i:-11 XN:i:0 XM:i:0 XO:i:1 XG:i:2 NM:i:2 MD:Z:124^TC26 YS:i:-15 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1362:7451 97 chr10 27853277 42 70M1D80M = 27853642 515 CCCTTCACTCTATTCTTGCTTGCCTACTTGCTGAGGAGACATCACATGTAATTCTTATCTTTGCTCCTCTGAGATAAAGTGTTTTTTTTTCTTTTGCCTTCTTGAAACACTTTATCTTTGATTTTTCTTCAGTTTGAATATGATATGCCT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????5?????????????????????????????????????5?? AS:i:-8 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:70^A80 YS:i:0 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1362:7451 145 chr10 27853642 42 150M = 27853277 -515 TCACTTCTTCGTTTTCTGTTTTTCCAATTTGAATATTTCTATTGACATTTCCTCAAACTTAGTGATTCTATCCTCAGTCATATTCAGTCTACTAACAAGGTCATCAAAGGCTCTTCATTTCTCTTACAGTGTTTTTGATTTCTAGCATTT ??????+??&?????????????????????????????5??????????????????????????????????????????????????????????????????????????????+??????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-8 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1045:28573 83 chrX 43928753 42 150M = 43928525 -378 CACTTTCTGGATTTCCAGTGGAAGTATTTTTGCCCAAAGGGGAATGAGTATTGTCAAAGTTGTCTTCGTGAATTGCACTTGAGGTCAAATTAAAAACAGCCTTTGGGGCTCAATTACAGAATCATAGAAATCATAGGCCTTGAAGGAAAA ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XS:i:-45 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:28573 163 chrX 43928525 42 150M = 43928753 378 CAGATTAGGCTCTTTGCATGGTTATATTTGATACATGCTGCAAACAGATCCACCAAGGAAGTTGAGTGCTGTTGGTTTTATTATTTTAAAAAAATCTGAAGGGGTCAGTTTACTTTTTCTTCTTCATCAGATTTCATAATAGTGGACAAT ??????????????????+?????????????5?5??????5????????????????????????????????????????5?????????????????????????????????????????????????????????5????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1479:32957 99 chr14 24281224 23 34M2I114M = 24281514 440 TGGGACTACAGGCATATGCCATCATGCCGGTCTATTTTTTTTTTTTTTTTTGAGATTGTGTGTCACTCTGTCCCCTGCCCTGGAGTGCAGTGGTGCCCACTTAGCTACATGCAACCTCTGCCACCAGGGGTCGAGCAATTTTCTGCCTCA ?????????????5??????????????'?+?5++?555??5??????????+?+?5?++?+?++??+?+?++???+???5???+?????+???+5++++??+?55+++++?+???5?5???+5?+???++?&???++++5??5???5?+ AS:i:-54 XN:i:0 XM:i:14 XO:i:1 XG:i:2 NM:i:16 MD:Z:28T25G1A2C10A5A18A0T7C0A0C13T6T2A17 YS:i:-33 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1479:32957 147 chr14 24281514 23 150M = 24281224 -440 TGGACCTCAACTCCTGACCACGTGATCCCCCTGCCACGGCCTCCAACCGTGCTGGGATTACAGCTGTTAGCCATCATGCCCGGCACCTGGCTAATTTTTTACTTTTATTTTTTGTAGAGATAGGGTCTCACTGTCCTGCTAGGGCTAGTC ++?+?++++?????5+++?+?++++?5++???5??+5+????5+++5&+?5??????+?????+++?+5????????????????????????????????????????????????????????????????????????????????? AS:i:-33 XN:i:0 XM:i:11 XO:i:0 XG:i:0 NM:i:11 MD:Z:3T1T1G11T8A6T8C1A0A15A3G82 YS:i:-54 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:12289 99 chrX 131659375 42 150M = 131659490 265 TATCATTATTAACACTTAGAAATCAGTTTATTCATCTCTAAAATTGGGTTAATTATGCACACACACTGGGTGTGTGGGAGGATTGAATGAGCTAATGCTTATAAAAGTGACATGTGAGCCCTAAAGTTCTCTGAAAAAGTGAAATCACCA ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????+ AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:12289 147 chrX 131659490 42 150M = 131659375 -265 GAGCCCTAAAGTTCTCTGAAAAAGTGAAATCACCATTCATATAAATTTTTAAAAATTCTACGAGCACTTTTATAGTTCTTATTGTGCAGCAATATTAACTCATTTAATCTTTGTAGCAACCCTATTGAGGTAGGTACTATTACTGTCCCC ??????????????????????????????????????????????????????????????????????5??????????????????????????????????????????????????????????????????????????+???? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:13510 83 chrX 22295426 23 57M1I92M = 22295146 -429 GGGGTTTATGGCCCCAGGCCAACCCCACGGGGCAAACACCGCGTCACTTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAATGGAACTCAGCCCTCAAATAGTTTTGTGTTGCACTTCAAGTCCAATTCCTTTTTCATGACT ++++++++++5++??5+5++?++??++?++??++?++++&5&+++++++++++++++??????????????????????????5????+???5?????????????????????????????????????????5??????????????? AS:i:-85 XN:i:0 XM:i:26 XO:i:1 XG:i:1 NM:i:27 MD:Z:0A1C0T0G0A0G2C2G3C1G2C0T2A2C0T2G0C4G0A3A0G3C0T3T0C1G92 YS:i:-3 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:13510 163 chrX 22295146 23 150M = 22295426 429 ACCCAGTCTCTAGTCTCAGTGGTCTACCATCCATTCTGTACCCAGCAACCAAGGTAATCGTGTAAGAATGGAACTCGGCCAGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGCAGGCCGAGGCAGGTGGATCACGAGGTCAGGA ???????????????????+????????????5?????????????????+????+?????+?5????+???????????5???????+????????????????????????5???????????????????????????????????? AS:i:-3 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:61T88 YS:i:-85 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:5854 83 chr11 9381847 40 39M9I102M = 9381550 -438 TGCACTCCAGCCTGGGCAACAGAGACTTCATCCCAAAACAACAACAATAACAACAACAACAACAACAACAACAACATTTTTACTGTGGTTAAATACACATAACACAAAATTTGCCATGTTGACCATTTTAAAGTATACAGCTCAGTAGTG ???????????????????????????????????????????????+?????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-32 XN:i:0 XM:i:0 XO:i:1 XG:i:9 NM:i:9 MD:Z:141 YS:i:-5 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:5854 163 chr11 9381550 40 150M = 9381847 438 CCAGAAGTATAATCATTAGGTCATAGGGCACATACATTTAAAGAAAAATTTTAGGCCAGCCACGGTGGCTCACACTGGTAATCCAAGCACTTTAAGAGGCCGAGGCAGGTGGATCACTTGTAGTTAGGAGTTCGAGACCAGACTGGCCAA ?????5????????????????+???????????????????????????????5?5??????????????????????+???????????+?????????????????????????????????????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:42C107 YS:i:-32 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1090:7106 99 chr17 46718616 42 110M2D40M = 46718964 498 CTCTAATTAAATTAATATGGAGCTTTTATCCACTGGGGCCTGGACCTTCCGCCATTTGTCACCTTGTCCAAAGCTATCTCATACCGAAAGAGAATTGAACGTTTCAGGAAACTTCCCCTTTGCAAATGCCCTTCTCTTGGGGAATGGGTA ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????5?????++????+?????? AS:i:-11 XN:i:0 XM:i:0 XO:i:1 XG:i:2 NM:i:2 MD:Z:110^AC40 YS:i:-3 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1090:7106 147 chr17 46718964 42 150M = 46718616 -498 TTGCCAGAGAAAAGACGGAGAATGACAAAGAGAGAAGTGAACTAGCTGGACTGGAGGCGGGAACTGGAGCGCAGCAGGCAGGTGGAGTGGCCAAAACCCGCGTGCTAAGAAGAGCCCACAGGGGCGGCAGGGGCGGCAGGGGCGGCAGAG ++????????++????????+?+???+?5????????????++5??5????????????????????5???????5?????????????????++???????+??+???????????+???????????????????????????????? AS:i:-3 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:41A108 YS:i:-11 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:11631 83 chr2 106914350 42 150M = 106914217 -283 AGGAAACTGGGGCCTGTTGAGAAGATGTTCACTAACATCTCCAGGATCTCACAGTGTGTTGGTGTCGGCCTGTGTGTGAGTCCGTTCTCACATTGCTACAAAGAAACACCTGAGACTGAGTAATTTATAACAACAAAAAAAGGAGGTTTC ?????????????????????????????????????????????????????5???????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-5 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:11631 163 chr2 106914217 42 150M = 106914350 283 ACTTCTTAAACCTGAGATTATTTTCATAGTATGTGCATGGGGTGTGAGAGGGAGCTGGGAGCAGACATCAGCTTGTTCAATCATCACAACAACTTCTGTGAAAGTATTTACTACTGTCTTCATCGCATAGATGAGGAAACTGGGGCCTGT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:68T81 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1181:22514 77 * 0 0 * * 0 0 TACAGTATATAATACATGCTTTGGGTACTTTGATATTTTTTGTACAGTATAGAATATATACCTTGGGTACTTTGATATTTTATTTACAGTATATAATATATGGTTTGTGAACTTTGATATTTCATGTAGAGTATAAAATATATATTTGGG ?????????????????????????????????????????????????????????????????????????????????????????????????????+???????????????????????+??+??????+??????????5??? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1181:22514 141 * 0 0 * * 0 0 TATACATTATATACTGTACATAAAATATGAAATTACATCAAATATATATTATATTAGGTACATAAAATACGAAAGTACATCAAATATAGATTATATACTGTACATAAAATATCAAAGTACCCCAATATATATTATATACTGTATATGAAA ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1045:4742 97 chr4 122562835 42 150M = 122563244 559 GAGGATTATTTTCAAAGTTTAATTCTATTTCACAAATCTCTTTTCCTCTTGGATTAATTAGACTGAACAAATATTTTTGTGTCAAAAGTTTAAATAAATAAAGTATGTCTAATCTACTCAATATGAAATATTCTTCTGCCATTAAAGATG ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????5???????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:2A147 YS:i:0 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1045:4742 145 chr4 122563244 42 150M = 122562835 -559 AAAGAGCAAGGGCAAACAGGAACCTGCTGTCAGGCTAGGATATTCCCCTACTTGCAAGCTGAAAGATTCACCTTCTATTGTTTTATGAATGTTGGCAGAAGACATAAGACACCTGGATCACAGATGAAGTACTTTGTTACTCACAATACA ??????????5??????5?????????????????5??????????????????????????+??????????????????????????????????????????????????????????????????????????????????????5 AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-5 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1036:2284 83 chr3 7459206 42 150M = 7459058 -298 CATGCTTTGGTGACTAGATTTTAAAACACTAGAACAGGCATGTTAGAAAGGGCACCCAGATGAGCAGGCTAGCTGAGTAGTCTGACACTGAAGATCTGCGCTGTGAATACAATGAATGTTTTTATCTGAGGACTGTAGAACATAGCCCCC ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1036:2284 163 chr3 7459058 42 150M = 7459206 298 TTGTTTTCCTTGTCCTTCCTTTTTCCAAATCCAACCATATGTACATCTTTCTTTGATAGTATTCCAAAAAATACTATTTAAAAAATCTTTACATGTAAGTATACTTCAATTTGGAAGTGAAGAATCAGTGGGAAAGGTAACCTGGTTGCA ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????5????????????????5??????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1036:31814 99 chr13 88893636 42 150M = 88893967 481 AATTGAAATCAATCAGTATGAAAAAAATTATAAGTTGTAAAATTGTAGTACATTTCAAAAAATTGCTCTAAGATTTAATTTTTACATTTCCATGAGAAGAAATAGAGGCTATACCTGAACTGTGCTTCTTTAAAAAAGATGACTTCTATA ??????????????????????????5??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:29G120 YS:i:-5 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1036:31814 147 chr13 88893967 42 150M = 88893636 -481 AAGAGATAATTTAGATGTTTTAAAATATAGTGATGAATTTCATTGAAAAGAGGTATTGTGTCCCCTAAGGTAATGATATAAGAAACAGTAGTAAATTTTTAAAACATTTGTGGCNCTTCTCTAGATTCTTACATTGTTCACATTTAGCNA ?????????????????????????5???????????????????????????????????????5????????????????????????????????????????????????#?+????????????+??????????????????#? AS:i:-5 XN:i:0 XM:i:3 XO:i:0 XG:i:0 NM:i:3 MD:Z:114A14A18A1 YS:i:-5 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1190:4085 99 chr3 106469909 42 45M4D105M = 106470163 404 GCATGACAGAGTGAGACCCTGTCTCAAAAAAAAAAAAAAAAAAAAAACAAAAGCTATTAAAAGTCATTTTTTTAATTGTGGACACAGCCATGCCTGTTAATTAAGAATAACAATTTAGAAAGGCCAGGAATTTTGTATTATAATTTGACA ?????????????????????????????????????????+???+?+??????5???+???5????????????????+??5+???5??+???5???????+?++?+??+??????+???+?5?+???+?+?????+?+???????+++ AS:i:-20 XN:i:0 XM:i:1 XO:i:1 XG:i:4 NM:i:5 MD:Z:45^AACT94C10 YS:i:-5 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1190:4085 147 chr3 106470163 42 150M = 106469909 -404 GAATTAGGACCCAACAAACTTTAGTTATACTTACTTGGAGGACGGCCTGATAATTTTGTGTTTTTACAAAATTCTTTATATTTATATATACAAACTTCTATCACCTAATACTGACTTGACAATCATAGGCCTATTTTAAAGACTTTTCTA ????5??????????????+??5??????????????????????????????????????????????????????????????????????+?+?????????????????????????????????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:100A49 YS:i:-20 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1814:34131 83 chr7 29940905 40 94M1D56M = 29940712 -344 CTCATAAGGGAAATTCCCTTTTATGGAATATTTGTAATAAAACCACTCTGAAAAACCCCGACTAACGCCCCGCCACACACTTAGAGCAGCCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAGACAGAGTTTGCCTTTCTGTTTTAG 5++??+5++++??+?++++??+++++++?+???++++???+?++?++++?+++?++??&?+?+??&5+??&?????++++??5+?5?55+'??++??????????????????????????????????????????????????????? AS:i:-48 XN:i:0 XM:i:14 XO:i:1 XG:i:1 NM:i:15 MD:Z:21A1G10G1T5G8C6T6G0T3G5T5C0T6A3^A56 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1814:34131 163 chr7 29940712 40 150M = 29940905 344 GCTCTTAAGTGCAGTAGGTGCTTGAGATTGAGGAGAGAGGTAAACAATATTTCTGCTGATGAGTTACAGTATCCCAAGTGTGAAAACCCCTGATTTGGTGAATCTGCAAAGCCCTTCTGTGGGAAGGGAGCCCATGTGAAGGACTACTTC ?????????????????????????????????????????????????????????????????????????????????????????????+??????????????????????????????+???+????5???????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-48 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1226:14638 81 chr7 48504200 42 134M1D16M = 48503789 -562 TCTTCTCAATTAAATTTTGTTTTTGTTTGAATGGTAAAAGTTCAGAAAGGGCCAGATTTAGGGAAGTAAAATAATTGGATGGAGATTAAGGATGAGGCCAATGAATTCAGTGCATTGTTAACATATCACAACTTGTTCGACACATCATTT ?????????????????????????+???????????????????????????????????????5???????????????????????????????????????????????????????????????????????????????????? AS:i:-8 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:134^G16 YS:i:-5 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1226:14638 161 chr7 48503789 42 150M = 48504200 562 AGTTTGAAGGCAACAAAAGAGGGATAGTTTAGGAAAAAGCATTTGAAGTTTGTGGTGACAAAGATACTTTTAAAAGAAGCTTGCATTTCCTAATTTGTTAGAAAACTGAAGGCTGTTTATACAGAGGTCACACTGCCTACTTATGAGACA ????????????+???????????+????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:104C45 YS:i:-8 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1036:4288 83 chr8 140147161 42 150M = 140147004 -307 AAGGCAGATGATCCCTCATGAGTGCATTGGGACCAAGACGAGGGGACTGAAGATAACTTGGAAGTGTTCAGAAAAGGATGGATGAATGACACTTGACTAGGGCAAAATGAGCACGAACAGTTCTATTGGGCATATGAGGTGGGAAGCACA ?????????????????????????????????????????????????????+??????????????????????????????????????????????????????????????????+?????+??????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1036:4288 163 chr8 140147004 42 150M = 140147161 307 TACAATATATCCATAGTGGCAAAACTAGCAGTGAAGGGTTGTTGAGTCTTGCACTTCCCATTCAGGGTTCACACAGGTTTGCAGGCTTGGGGCTCCCTGGGGTTGGTGCTCTCCTCTACTCAGATGTGCTCCTTTGTAACAGGAAGAATA ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:8750 83 chr15 62098321 42 150M = 62098071 -400 TAGTCTGTTTTCTTTTCCCTATAGGTTTTCTTTACAGCTGTTTTTTTCTTTTTCAACTTAAAAGAGTGTATTTAATATTCCCTTTTAAGATAACATGATAGCCATGAAGTCTTGTATGAGAAAGGTAGGAGGGAAGTTAATCTAGAGAAT +????????+????????????5????????????????5?????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:77C72 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:8750 163 chr15 62098071 42 150M = 62098321 400 AAGGATGATATCTATGTATCAATCAGTATGTCAGCCTGAAATAATCAAACAGGTGGGAATCCAGTTTTAAAGAGAGCATTTAAGCAAATAGCTGGGAATGACCATCCAGGAGAGAAAGACTCCAGAGAAATGGGGTCAGTGCTCTGAAGT ??????????????????????????????????????????????????????????????????????????????????????????????????????????????+??????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-5 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:36479 99 chr1 210979448 42 150M = 210979773 475 GGTTACTAGGTTTAACATGTTGGAGAAATTGCAGAATTGACTTCCAAAGTAACTGCACCACTTTACATTCCTGCCAGCAGTCTATAAGAGTTCCAGTGTCTCAATATCCTCATTAACAATTGTTATTGTGTCTTTTTCAATTATCACCTT ???????????????????????????????????????????????????????????????????????????????????+????????????????????????????????????????+????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:40T109 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:36479 147 chr1 210979773 42 150M = 210979448 -475 ATATAATCTGGATACAAGTCCCTTCTCAGATAAATGATTTGAAAATACTTTCTCATATCCCATGGGTTGTGGTATCATTTTTGGTGGTATCATTTACAGCACAAAAGGTTTTAGCTTTGATGTAGCTCATTGTACTTATTTTTTTTCTTT ?????+????????????????????????+??????????????+?????????+?????????????5???????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-5 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1624:21684 99 chrX 66641527 42 150M = 66641837 461 GTCCTTGCAGAGAACATAAACTCATTCTTTTTTACCACTGAATAGTATTTCATGGTTATATGTGCCACATTTTCTTTACCCAGTCTAATATTGATGAGCATTTGGGTTGGTTCCAAGTCTTTGCTATTGTGAATAGTGCTGCAATAAACA ??????????????????5?????????????????????????????????????????????????????????????????????????????????????????????????????????????????+?????????+??????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-8 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1624:21684 147 chrX 66641837 42 4M1D146M = 66641527 -461 GTGTAAAAGTGTTCCTGTATCTCCATGTCTTCTCCAGCAACTGTTGTTTCCTAACTTTTAATGATCACCTGCTAACTGGCATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTAATGACCAGTGATGATGAGCTTTTTTCAT ?????????????????+???????????????????????5?????+????++??????+?????+?????????????????????????????????5?????????+?????????????????????+????????????????? AS:i:-8 XS:i:-80 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:4^A146 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:16673 99 chr1 23738122 42 150M = 23738356 384 ACAGGTTTTCCTCTGCCTAGCAAGCTCACTTCAAGGACAGTTATAAGATAATGCTGTTTGGGAAACCAAGGCCAAAGGAATGGGCTCCAGACACCCCTCCCCTCCAGAGCAAGGTTGAAGGAAAAAAAGAGAGAAAGACAAATTCCTTTA ??????????????????????5???????????????????++??????+????????????+???????????????????????????????????????????????+??????????????????????+?????+?+??????? AS:i:0 XS:i:-84 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:16673 147 chr1 23738356 42 150M = 23738122 -384 CAGCTACAAGGCTGCCAGCTATGCAAGGCCACAAGTTATGCTATGCTATAGATTATGTGACCTATCATATGATTAACTGCTTTTGTTTTGCTTCTGTAAGCCTGCTTGTAAAAACCCCGCTCTGTCTTTGCTAAAATGCTCAGCTGTTTA ????????????????????????????????+???5?5???????????+?+?????????+????+?????+?+?????????????????????????????????????????????????????????????????????????? AS:i:0 XS:i:-62 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1081:7654 97 chr20 37402373 42 150M = 37402778 554 TAAAACCACACTCAATACCCATCAACTCCCTTCCCCCTGCCTCTGTCCATCCCCTACTCTTTGCAACCACCATTCTACTTTGTAAGAGTTTTTCTTTAGATAACTCACATAAGAGAAGCCATACAGTATTTGTTGTTTTGTAACTGGCCT ????????????????????5????????????????????????????????????????????????????????????????????????????????????????????????????????????+??????+????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:55G94 YS:i:-14 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1081:7654 145 chr20 37402778 42 135M1I14M = 37402373 -554 ATCAGATGGTAGTTCTATTTTTAATTTTGTGTGGAACACTCGTACTGTTTTCCATAGCAGCTGCACCATTTTCATTCCCACCATCAGTGCATGAGAGTTCCAGTTCCTCCACATCTTCACCAACTCATTATGCTGTTTTTTTTTTTTTTG ????+?????????+5??????????+?+5???????????????????+????+???5??5?????????+?????????????????????5???+?????+??????????????+??????????????????????????????? AS:i:-14 XN:i:0 XM:i:2 XO:i:1 XG:i:1 NM:i:3 MD:Z:4T23T120 YS:i:-5 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1054:36605 83 chr2 107025610 6 150M = 107025407 -353 TTTGCTCTGATGGGCCCTGTTTCCTTCAGTGCCAGGAAGAGTGGCTCTCAGACCTTTCTTCTAGCATCCCTCATGTATGCAGTGTTGGTTATTATTTTCAAGGCCTTGGTAACAAAATGCCCCTAGTGTGACCAGTCAGACTGACAGTCC ??????????????????????????????????????????????????????????????????????????????????????????????????????????+??????????????????????????????????????????? AS:i:0 XS:i:-11 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:36605 163 chr2 107025407 6 150M = 107025610 353 GTTGGGCTTACAGGTGTGAGTCACTGAGCCCAGCTTGTATTCTTTTGATTTTAAAAAAATATTCTGAATGTGATCCACCAAATTAATTTCACCATCCACTAATAGGTAGCAACCTGAATGAAAAATACTATTATAAACTTTACACAAAAC ??????????????????????5???????????????????????????????????????????????????????????????????????????????????????????????????????????????+??????????????? AS:i:0 XS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1172:22529 77 * 0 0 * * 0 0 TACAGTATATAATACATGCTTTGGGTACTTTGATATTTTTTGTACAGTATAGAATATATACCTTGGGTACTTTGATATTTTATTTACAGTATATAATATATGGTTTGTGAACTTTGATATTTCATTTACAGTATAAAATATATATTTGGG ???????????????????????????????????????????????????????????????????????????????????+?????????????????+???????????????????????+??+????????????????55??? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1172:22529 141 * 0 0 * * 0 0 TATACATTATATACTGTACATAAAATATGAAATTACATCAAATATATATTATATTAGGTACATAAAATACGAAAGTACATCAAATATAGATTATATACTGTACATAAAATATCAAAGTACCCCAATATATAATATATACTGTATATGAAA ?????????????????????????????+????????????????????????????????????????&??????5???????+?????????????????????????????+???????????+???+?????????????????? YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1362:4476 99 chr6 143836666 42 89M2I59M = 143836941 425 GGTGTCAGCAGAGAGGGGAGAGCTGCCAGGCTGTGGCCCCACGCCAGCCTCTGCCATAGGGCTGTGTAATCCTGGGGAAGGCGTTCAACCTCTCTGTGCCTCAGTTTCTTCACCTATTAAATGTGAGTGATAATCGTGCATCTCCCTCCT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-11 XN:i:0 XM:i:0 XO:i:1 XG:i:2 NM:i:2 MD:Z:148 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1362:4476 147 chr6 143836941 42 150M = 143836666 -425 TCCCATTTAACCTGAAGTCCTAAATGAGAATCAGGACAGAAATGTCCTGATTAGGACTCAGGCTAACTGGCAAGTGTAGCAAGGTACAGCCATGTTAGACAGATGGAGAGGCTGCTTTTTACCTTTCCTGGGGTGACCTTGATATTCGAG ????????+????????????++????????????????????????????????????????5??????????????????????????????????????????????????????????????????????????????????5??? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-11 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:2002 99 chr15 56749591 23 150M = 56749940 499 ACGTACCTATTGGAATGGCTAAAACAAAAAACAGTGCCAACACCAGATGCTGGCAAGAAACTATCACTCATATATTGCTGTGTGGGAATGTAAAATGGTACAGGCATTCTGAAAATCAGTTTTATAGTTTGATATAAAACTAACATGCAG ??5????????????????????????????????????????????????????????????????????????????????????+?????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-83 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:2002 147 chr15 56749940 23 150M = 56749591 -499 TATTTAATGGATGCTTTAATTAATTTTCATGCAATTGAAATAAAGCTGGGAAAAATCCCAAGACCCTTTATATTAAAAAAAAAAAAGCCTCCATGCAATTATCACACAACTCAACAATTTTACCTCTTAAGCATTTATCCCAGGGAAATG ?+??5??+5?+55+++??+++++?++?+??5?+?++?+?++++?5+????+??++?+++?5++5?+?+5+++?+????????????????????????????????????????????????5??????????????????????????? AS:i:-83 XN:i:0 XM:i:25 XO:i:0 XG:i:0 NM:i:25 MD:Z:0G1G2C1A2C3A0G3G1C2G0A6C1C2C1C1C0C7C2C1G0A0G9G1C1C78 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:33677 97 chr8 139659024 40 150M = 139659414 528 TGATTTTCCACTGGGACTTCCGTCAGCTACCCCTGCCCCTAGCCTGTCTGAGCAAAGGGTATAGGCAAGTGGTAGTACTGAGTGTGGCTGTGCAACCATGGCATGACCCACCATTAATATGGTTAGTTGGTGTGTCTCCAAGATAGCCTG ????????????????????????????????????????????????????????????????????????????????????????????????????????????????++???????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-50 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1045:33677 145 chr8 139659414 40 134M12I4M = 139659024 -528 AGCTCTGCAGAGAGGCCCACCCAGGTGGTAAGGAACTGAGGTCTCCATCAACAGCCACAAGAGTGAACCTGGATGGAAGGGGATCTTCTACCAGATGGACCCTGAGATGACTGCAGCTCAGGGCTTGAGGGTTGCCTCATGAAAGACCNG ????????????5??????????+????????????????????????????????+????????+???5??????????????????++?????????????????????????????????????5???+????????????????#? AS:i:-50 XN:i:0 XM:i:3 XO:i:1 XG:i:12 NM:i:15 MD:Z:23T112T0C0 YS:i:0 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1696:31923 99 chr19 30176666 42 150M = 30176935 420 AAGTGTGTTAGAGTGGCCGGGCACGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGAGGCCGAGGTGGGTGGATCACGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGTT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-8 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1696:31923 147 chr19 30176935 42 24M1D126M = 30176666 -420 GGCGATAAAGCGAGATTCCATCACAAAAAAAAAAAAAAAAATGTGTTAGAGTCTCTCATATCTGTGGCATCTGCATCTTTTCCAGCACTAGGAAGATTCCCAGCAAAGAGGGGCCACACTGTTTGGAAATACACGTGCCTGCCCTTTTTA ??5?????????????????+????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-8 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:24^A126 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1805:10692 83 chr16 56316819 42 37M4D113M = 56316495 -478 GTATGAGTCTCTCCAGGCCCCTCAGGAAGAAATTGAGAAGACCGGCGATCAGTGTTCTGTCCTTAGTTACCACTCTTCTGAGGTCTACTTGACTGCAATGGGCATTTTCCATCTGGTGGGGGTCCAGGTTTCTGAAAAACAACTCAGGGA ??????5???????????????????????????????????????????????????5?????????????????????5????????????????????????????????????????????????????????????????????? AS:i:-17 XN:i:0 XM:i:0 XO:i:1 XG:i:4 NM:i:4 MD:Z:37^AAGA113 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1805:10692 163 chr16 56316495 42 150M = 56316819 478 CAAAAGACTGCGTTTATCTGCAGGAGGCTGAGCAAGGAGAGTCAGGCAGCTCATGCTTAACATCTCACTTCCTCAATGGCCCTACAAGCAAGGGCTTTTAAAGGCAAGGGTACATTTTAGGAAAGCAGAAGCTACAGGCAAAACCGTAAA ???????????????????????????????????????????????5??????????????????????????????????????????????????????????????????????????????5??+???????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-17 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1217:22263 99 chr1 178767019 42 102M1D48M = 178767141 272 AATCTGTTCTTCATCTTGTTGAACTGTGAGAATCCACTCATACACATATTTTTTTCAATAAATATTTTGGAAATTTTGTCTTGAAGATTTCCAACAATTTGTAAAAAAAAAAAAAAAAAAACAACATAGCTTAGAAATATAAAAAAAAAT ???????????????????????????????++?++?????????????????????????????????????????????????????????????????????????????????????++?+???+5??+++??+??+????????? AS:i:-11 XN:i:0 XM:i:1 XO:i:1 XG:i:1 NM:i:2 MD:Z:102^A20C27 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1217:22263 147 chr1 178767141 42 150M = 178767019 -272 CCACATAGCTTAGAAATATAAAAAAAAATTAAGAAAAAGGGATGGCGTGAATGTATAAAATATATGTAGATACTAGTCTATTTTGTCTTTTACTACCATGAGATATATCCAAATGTATTAAGAAAAGTTAATATTTATCAAGACTTATGC ????+5????5??????????????????5???????5???????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-11 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:22138 99 chr12 104200282 42 150M = 104200577 445 TTCCTGAAAGGGTTGCTTGTTAATTAATGTGCTGTACAGCTCTTTAAAGAAATGGCCTATCTGCAAAAGACAGGGGAAAAAAATAGATATGGCAACTGGTTCCTCCTCCAAACACCAAAATGACTACTAGAAATAAACAGTACCTCCTAA ??????????????????????????????????+??????????????????????????????+???????????????????????????????????????????+??????????????????????????????????????+? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:22138 147 chr12 104200577 42 150M = 104200282 -445 TACATTTCATTTCCAAACATCATTAAACCATAGAAAAACAGGAGAAGTAAACCTACCCGCTGTACATCATAATGAAGTCCACGAATTGCAAAATTTGGGTCATACTCATACTTCCTGATTTCTGCTGGATACTAAGAAAGAAGAAAGGAA ???+??????????????????????????5???????????????????+??????????5?????????+?????????????+??????????????????????5????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:26725 99 chr12 118162116 42 150M = 118162325 359 TCAAAAAAAAAAAAAAAAAAGAAAAATAACATAAATCTACCAAACAATACCTAAAAATAAGTAGCAATGTTCCTCAGAAACTGAGGTGTTCGCCCAGGTACTGATCCACCATTTACAAATATAAAAACTCCACTTGTTCTAAATAATGAT 5???????????????????5+?????????+?+?+?5??+???++?????5????????5??????????++5??5?+?5????+++??+????????+5??????????+???5???????????55+?????+?????????????? AS:i:-21 XN:i:0 XM:i:7 XO:i:0 XG:i:0 NM:i:7 MD:Z:33T11T32T11A8T29A5T14 YS:i:-2 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:26725 147 chr12 118162325 42 150M = 118162116 -359 TTGCTGCTGGTAGCCACACAACACGCATCATCTCTCTGGATCATCAGCAAAAAAACCTGCCCCCCCACCCCCGGCCGCCTGCAGTTCTGGACTGTTGGATGCTCCATGATCCACATCAAACAGGCAGGGGGTCTGTGCAATCCCAGACAT ???????????????+????+?+&?????????????5?+?????????????+?5????????????5??????????????5????????????????????????+??????????+?????????????????????????????? AS:i:-2 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:23A126 YS:i:-21 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:21997 99 chr1 40603535 42 150M = 40603798 413 TCTCTACTAAAAATGCAAAAAAATTAACCAGGCATGGTGACGTGCACCTGTAATCCCAGCTACATGGGAGGCTGAGGCAGGAGAATTGCTTGAACTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCACACCACTGCACTCCAGCCTGGGC ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:21997 147 chr1 40603798 42 150M = 40603535 -413 AGATGTTTGACATCACTAATCATTAGGGAAACGCAAATCAAAACAACGAGCTACAATCTCATACGCATTAGGATGGCCACTATCAAAAATATGAAGAAAATAACAAGTTTTGGTGAGGATGTGGAGAAATCAGAATCCTTGTGTACTGTT ????????????????5????????????????????????????+????????????????+??????????5??????????????????????????5????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1036:15092 99 chr18 49116658 42 150M = 49116894 386 TACTTATTGTGAATAAAGAGAGTTGACTGTATTTATTTATTTCTTTACAATTAGTCCTTTCTTAGCCAAACTCCAAGAGAATATATATATTGTCATCTTGTTTTCTGCTGTCTCCTTGGCATTTAGAACAATGTCTGGGACACAGCATTT ?????+?????????????????????????????????????????????????????????????+?????????????????????+??+????????????????+???????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-1 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1036:15092 147 chr18 49116894 42 150M = 49116658 -386 AGAGTAAATAAAGACACTGTCAGTCTGTATATACAATATTAGAAAAGCTTAATTTTCATACTTTAGATAAAAATTAAATAAAAATATAATATCTATTTTTTTCTGTATCCCAGTATCTTATTTTTGTGTAACTGTGAAGAATATGTTCNT ?????+???????????+??????????????+??????5?????5?????????????????+??????????????+???????5?????????+??????????????5????????????????????????????????????#? AS:i:-1 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:148C1 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1787:6026 97 chr18 109959 0 138M1D12M chrUn_gl000225 81262 0 CTATGGAGGCATAGTGATATATCTCTGCACTGATCACTCATGTGATGTAACGCTTCTCTAAGCTGCACCTGAAGGGGAATTGTGACAGATATCTCCACTGATCACCCAGGTGATGGGACTTTTGTCTAGGCTCTGCCTCTGGGGGTAGTT ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????+??????++5?? AS:i:-82 XN:i:0 XM:i:15 XO:i:1 XG:i:1 NM:i:16 MD:Z:6G10C37G10G3A4C39T0A3C10C1C0A3^A1A7T1G0 YS:i:-60 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1787:6026 145 chrUn_gl000225 81262 0 150M chr18 109959 0 ATTGTGACATATCTCTGTACTGATCACCTAAGTGATGTAACACTTGTGTAGGCTCTTCCTACAGGGGCATTTTGACATATCTCTGCACTGTTAATCGAGGTGATGTAACTCTTTTCTAGTCTGTGTCCTCAGGGGTATTGAGACATATCT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-60 XN:i:0 XM:i:12 XO:i:0 XG:i:0 NM:i:12 MD:Z:17C24G13G12C0C23C14A3G5G5C2A6G14 YS:i:-82 YT:Z:DP +A00217:76:HFLT3DSXX:1:1101:1732:31829 99 chr19 30176666 42 150M = 30176935 420 AAGTGTGTTAGAGTGGCCGGGCACGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGAGGCCGAGGTGGGTGGATCACGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGTT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????+???????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-11 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1732:31829 147 chr19 30176935 42 23M1D127M = 30176666 -420 GGCGATAAAGCGTGATTCCATCAAAAAAAAAAAAAAAAAAATGTGTTAGAGTCTCTCATATCTGTGGCATCTGCATCTTTTCCAGCACTAGGAAGATTCCCAGCAAAGAGGGGCCACACTGTTTGGAAATACACGTGCCTGCCCTTTTTA ??'+?+??5555+++??++?++?+??????????????????????5??????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-11 XN:i:0 XM:i:1 XO:i:1 XG:i:1 NM:i:2 MD:Z:12A10^C127 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1353:10347 83 chr10 42599967 0 86M1D64M = 42599631 -487 AATCCTTGGACTCGAATGCAATCATCATCGAATGGAATCGAATGGAATCATCGAATGGACTCGAATGGAATAATCATTGAACGGAACGAATGGAATCATCATCGGATGGAAATGAATGGAATCATCATCGAATGGAATCGAATAGAATTA ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-48 XS:i:-43 XN:i:0 XM:i:8 XO:i:1 XG:i:1 NM:i:9 MD:Z:5A4T28A46^T0T25C10G19G4C1 YS:i:-48 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1353:10347 163 chr10 42599631 0 103M1D47M = 42599967 487 ATGGAAATGAAAGGAGTCATCATCTAATGGAATTGCATGGAATCATCATAAAATGGAATAGAATGGAATCAACATCAAATGGAATCAAATGGAATCATTGAACGAATTGAATGGAATCGTCATCGAATGAATTGAATGTAATCATCGAAT ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????5??? AS:i:-48 XS:i:-53 XN:i:0 XM:i:8 XO:i:1 XG:i:1 NM:i:9 MD:Z:33C15C9C11T5G8T7A8^G35C11 YS:i:-48 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1841:19836 99 chr2 7930988 24 150M = 7931246 400 AAGGTTCTAATGGGAGAGAACTCATACACTTAGGCCTGTGTTCCCCAAGGCATGCTCCATGTATCTTCTGCATCAGACTCATCTGAATGTTATTGAAATGGACGTTTTGAGAGTCTACTCCAGACCTGTGGAATCAGAATCTCTACAGGC ??????????????????????????????????????????????????????????+?????????????????????????????????????????????+????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-71 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1841:19836 147 chr2 7931246 24 128M10I5M2D7M = 7930988 -400 GGTTGCAATTTCAAAAATAAGAAATGCAAGAAAGGGATTTTGTGCAGAAGGAGCAGTAAGGCACCGCCGGGCGCGGTTGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGAGAATCACTTGAACCTGGGAGGTGGAGGTT ???????+?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-71 XN:i:0 XM:i:5 XO:i:2 XG:i:12 NM:i:17 MD:Z:117G2C1G10^CA4A1C0 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:9878 83 chr8 107273782 42 150M = 107273485 -447 TGGCTGTCTCTGTCGAGTCATAAAGGTCACCAAGGAAGTGGGGGAAGGCTGGCAGTCATAGGCCTCACCCCATTCCCATGGAGCTGGTAGTCCTAAAGGCCAGTCTCACTACCGCCGTGCCCCCTGCCGCAACAGCACCAAGTGTATTTC ?????????????????????????????????????????????????+??????????????????????++???+??????+????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-6 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1063:9878 163 chr8 107273485 42 150M = 107273782 447 AGGAGGTGGCACTTTCCAGACACCATCCACTGCAGTCCTATAGGAAGGATGTAAACTTACCCTAGGAAAACCTGACTAAGTATTCAGGTGTCTCAGGTGGTGGACAGGACCATAGAGCTCCCAAAGGATTATGACCTTTGTCTTTGGCTA ????????????????????????+??++???????????+????+??+??????????????5???????????55+??++?+?????+????+??+?????+?????????+?????????+??????????????????????5??? AS:i:-6 XN:i:0 XM:i:2 XO:i:0 XG:i:0 NM:i:2 MD:Z:27A61T60 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1796:2440 99 chr3 106469909 42 45M4D105M = 106470163 404 GCATGACAGAGTGAGACCCTGTCTCAAAAAAAAAAAAAAAAAAAAAACAAAAGCTATTAAAAGTCATTTTTTTAATTGTGGACACAGCCATGCCTGTTAATTAAGAATAACAATTTAGAAAGGCCAGGAATTTTGTATTCTAATTTGACA ???????????????????????????????????????????????+??????????????5?+??????????????????+?+???+????5??+??????????????++?++????????????++????++?+??????5???? AS:i:-17 XN:i:0 XM:i:0 XO:i:1 XG:i:4 NM:i:4 MD:Z:45^AACT105 YS:i:-5 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1796:2440 147 chr3 106470163 42 150M = 106469909 -404 GAATTAGGACCCAACAAACTTTAGTTATACTTACTTGGAGGACGGCCTGATAATTTTGTGTTTTTACAAAATTCTTTATATTTATATATACAAACTTCTATCACCTAATACTGACTTGACAATCATAGGCCTATTTTAAAGACTTTTCTA ?????????????????+?????????????????????????????????????????????????????????????????????????????????????????+?????????????????????????????????????????? AS:i:-5 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:100A49 YS:i:-17 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1371:4773 99 chr6 143836666 42 89M2I59M = 143836941 425 GGTGTCAGCAGAGAGGGGAGAGCTGCCAGGCTGTGGCCCCACGCCAGCCTCTGCCATAGGGCTGTGTAATCCTGGGGAAGGCGTTCAACCTCTCTGTGCCTCAGTTTCTTCACCTATTAAATGTGAGTGATAATCGTGCATCTCCCTCCT ?????????????????????????????????????????????????5????????????????????????????????????????????????????????????????????????????????????????????????5??5 AS:i:-11 XN:i:0 XM:i:0 XO:i:1 XG:i:2 NM:i:2 MD:Z:148 YS:i:-12 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1371:4773 147 chr6 143836941 42 150M = 143836666 -425 TCCCATTTAACCTGAAGTCCTAAAGGAGAATCAGGACAGAAATGTCCTGATTAGGACTCAGGCTAACTGGCAAGTGTAGCAAGGTACAGCCATGTTAGACAGATGGAGAGGCTGCTTTTTACCTTTCCGGGGATGACCTTGATATTCGAG ????+??5???????5??5?+?+?5????+????????????5????5?+?????????5???+???5???+???5+?????????????????????????????????????????+?+???????+??????????+??????5?5? AS:i:-12 XN:i:0 XM:i:3 XO:i:0 XG:i:0 NM:i:3 MD:Z:24T103T3G17 YS:i:-11 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:6073 99 chr9 90199855 42 150M = 90200067 362 GTTCTGGGATTACAAGCGTGAGCCACTGCGTCCAGCCGTATATCCTCTTTTGAGAGTTGTCTGTTAAGTCCATTGCCCATTTTAAAATCAGGTTATTTGTTTTCTTGCTTTGGAGTTGAGTACCATGTATATATTTTGACTGTTCACTCA ????????5???????????????????????????????????????????????+??????????????+????????????????????++??+???+????+???????????????????+????????????????+??????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1054:6073 147 chr9 90200067 42 150M = 90199855 -362 TATTGTTTCCTTTTCTATGCAGAAACTTTTTCGTTTGATGTAATTCCATTTGTCTATTTTTGCTCTTGTTGCCTGTGCTTCTGGGGTCATATCCAAAAAGTCATTTCCCAGACCACAGAAATCTTGATGCTTGCCCTTTTGGAGCCATAA ???55???????????+??????????????????5???????????????????????+????????????????????????????????????????????????????????????????5????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:29011 83 chr12 49966033 42 150M = 49965814 -369 TAGAACAGTGGTCCCCAAGCATTTTGGCACCAGGGACTGGTTTTGTGGAATACAATTTTTCCACAGACAGGGGGTTGGGGAACATGGTTTCAGGATGAAACTGTTCCACCTCAGATCATCAGGCATTAGATTCTCATAAGGATGGTGCAA ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????5??????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:29011 163 chr12 49965814 42 150M = 49966033 369 CTGGGGAGTCATCATCAGGAAGATCTGGATGTGGATGCTTAAGGAGAGAGTGTGGATAAGAGAATGAGGAGGGCTCAAGACCATTGCATGGGACATGCCAACATTTAGGGCAAGGCAGAGGAAGAGGGGCAAGAAAATAAACCAAAAGAA ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????+??????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:10504 99 chr16 86977717 42 150M = 86977977 410 CAGAGCAAACGCCACCTCTTGGGTTATATCTCCCTGGTTACCGCCAGGCCATTCCTCGTACCTAGACTGGGGGACATGTGCTTGATCTTGAACTCACTTGAACACCGCTTCTGCTCCATAGATTCTGCCCTTCTAGAGTGTAGGGCCATG ???????5???????????????????????????????????????????????????????????????????+?????5???+????????????????????????????????????+????????????????????????+?? AS:i:-3 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:122C27 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1045:10504 147 chr16 86977977 42 150M = 86977717 -410 CATCTAGATATGACCTCTCACCTGCCCATAAAATGAAATGAAATCCATAGGACAAAATTTGACCATGAATTTATTCTCATTCTGCATCCAGCCTTTTGAGTGATAATAATAATAATAGCCCATATTTACCGACTCTTGTTTTGGCTGGGT ?????????+??????????????????????????????????????????????????????????+????????????????????????????????????????5?????+5????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-3 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1226:16517 153 chr14 103677731 24 11M3I136M = 103677731 0 ACGCTCATCCGATCACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCCGGCCACATTGTTCTTTTTCAAGGTTACTTTGGCTACTCGAGGTCCCTTGAGAGTCCATATGAATTCTAGGATGGATTTTCTATTTCTGCAAAAA +55?+?+??&?++++??????+????????+??5?????+?????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:-32 XN:i:0 XM:i:6 XO:i:1 XG:i:3 NM:i:9 MD:Z:0G1C1G1T2A1C135 YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1226:16517 101 chr14 103677731 0 * = 103677731 0 CTCCCAAAGTGCTGGAATTACAGGCGTGAGCCACCGATCCCGGCCCATTGTTTATTATTAAATTTTGGATTCTAACCACGAGTAACATATATATTACCTTGTTATTCTTTTTTTTTTTTTATATTTATGCTAAATTGATAGGAAGAGCTC ????????????????????????????????????++???????+++5++????????+??+?5?++?+?+?+++?+?&++?+??+??+?++??+?+5++++?+???+?++?+++????+?+??++??55++?++?+++?+?++?5?5+ YT:Z:UP +A00217:76:HFLT3DSXX:1:1101:1416:4946 83 chr3 148379908 42 150M = 148379791 -267 TCAAAAGGCAACTTACTACCTCTATACATGCTTATTTGTATTTGTTACATAAATACTAATGAGGTATCTAATCAATTGTAAAATCTATAAAAGAAAAAGGAACACAGTTGAAAAATATCAGGTTTCTTTTTTACAAATTGATCATAAAAG ???????????????????????????????????????+?????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-8 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1416:4946 163 chr3 148379791 42 104M1I45M = 148379908 267 TATTTTAGGAATTACATATTTTTAAGTATTAATTCTTTAGCTAAAGTTTTATTTAGCTAAACCATGATTTTTTTAATACATGTAAGTTTAAGGATGAAAACATGAAATGTCCCTTGTTTCAAAAGGCAACTTACTACCTCTATACATGCT ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????5???????????????? AS:i:-8 XN:i:0 XM:i:0 XO:i:1 XG:i:1 NM:i:1 MD:Z:149 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1027:6903 83 chr3 115842180 42 150M = 115841986 -344 TTTGTTTAGAGATACAGTTTATCCTCTGCATATATTCTGAACTTAACTTGTGCTTCTCACAATGCATATGAAAAAAATGGCAACAAAAGTCTAGGTTTTTCAATTTAGATGAGATTCCAACAATAGGCAACATTATGCCATATGGAAAAC ?????????????????????????????????????+??????????????????????+??????+?????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-2 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1027:6903 163 chr3 115841986 42 150M = 115842180 344 ANTTATTAGTGTTGNATCATGTCTCCTCTACCCACATGTAAACAGATATAACCAAGGGTGGAGTGAGCTGCCTGCTGCCTTCTCATCTGGGTATGTGTGATCTGTGTCTTAAAAGAAGTTTTGGGAAAGTGGCCAAAGGCCACCAGAGGA ?#?????+??????#?????????????????????????????????????+??+??????????????????????????5??+???5??5???????????????????????????????????????????????5?55?????? AS:i:-2 XN:i:0 XM:i:2 XO:i:0 XG:i:0 NM:i:2 MD:Z:1A12G135 YS:i:0 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1027:13228 83 chr10 111225891 42 150M = 111225606 -435 TTCAGTATTTCCCAAGCTATATCTTAGAAATTTTGTATCAGAAGCCTCCACAAATCTATCCTATAAATCAATGTTCTCAGATCTTATGTGTATCAAAATCATGTGGGTATAGTAGAGACAATTTGTCTCAACTCAAGTTCTATTTACACC ?+????????????????????????????????+?????????+????????????++???????????+????????????????????+?????????????+???????????+???????????????????????????????? AS:i:-3 XN:i:0 XM:i:1 XO:i:0 XG:i:0 NM:i:1 MD:Z:1G148 YS:i:-11 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1027:13228 163 chr10 111225606 42 150M = 111225891 435 GNAAAATCCCATNTNTACTAAAAATACAAAAATTANCNGGGCATAGTGGTAGGAACCGTNATCCCAGCTACTTGGGAGGCTGNNGCAGGAGAATTGCTTGAACCCANGAGGCAGAAGTTACAGTGAGCCNNGATCACACCACTGCACTCC ?#????5?????#?#???????????5????????#?#?????????????????????#???+?????????????????+##??????????????+????5??#???5??????+??5???????5##+???????5?+???????? AS:i:-11 XN:i:0 XM:i:11 XO:i:0 XG:i:0 NM:i:11 MD:Z:1C10C1C20G1T21A22A0G22G22A0A19 YS:i:-3 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1334:9408 83 chr14 41629660 42 150M = 41629384 -426 ATAGGATCTCAAGTGCAGTTTAGTGCTCTCTTTAAAAAATTTCTTAACTTTATTTTCTCACTATAACATTGTCATACAGGAATAAACTACAGAGTAAATGAATCAGTAGAACTTGATGTTCTGGTCACATAGACACTAAAAAAAGGTAAA ?????????????????5???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YS:i:-11 YT:Z:CP +A00217:76:HFLT3DSXX:1:1101:1334:9408 163 chr14 41629384 42 80M1I69M = 41629660 426 TGCAGCACACCAACATGGCACATGTATACATATGTAACAAACCTGCAGGTTGTGCACATGTACCCTAGAACTTTAAGTATAAAAAAAAAAGATAGAAAAGAAAAATAGATGAACATCAAATATGAATACAAAAAATTGTATATTAACATC ??????????????????????????????????????????????5??+????????????????????????5????+5?????????++?5++???+?????????????5+??5??++???????+????????+??++???5?+? AS:i:-11 XN:i:0 XM:i:1 XO:i:1 XG:i:1 NM:i:2 MD:Z:140C8 YS:i:0 YT:Z:CP diff --git a/testdata/hg38ToHg19.over.chain b/testdata/hg38ToHg19.over.chain new file mode 100644 index 0000000..d5db4cc --- /dev/null +++ b/testdata/hg38ToHg19.over.chain @@ -0,0 +1,236543 @@ +chain 20849626768 chr1 248956422 + 10000 248946422 chr1 249250621 + 10000 249240621 2 +167417 80249 50000 +40302 288020 253649 +1044699 2 1 +3716 3 0 +1134 4 0 +4 10 0 +3377 1 0 +7258 1 1 +27 1 1 +2083 1 0 +209 0 6 +307 1 1 +31 1 1 +322 3920 0 +710 1 1 +52 0 8 +70 1 1 +208 1 1 +44 1 1 +1672 1 1 +25 4 0 +1990 1 1 +44 1 1 +233 0 11 +905 1 1 +48 1 1 +1293 1 1 +25 1 1 +2304 1 0 +641 22 1 +1014 12 12 +1038353 64892 50000 +2716 2 1 +5539 11 11 +1747 0 1 +1084 4 0 +3732 0 2 +2453 0 10 +29 0 10 +185399 1 0 +958314 6505 150000 +1324 0 1 +3430184 0 1 +15819 1 0 +1121911 1 0 +1037710 1 0 +844703 1 0 +1971071 1 0 +6015 1 0 +12423 1 1 +39 1 1 +1404 1 0 +4091 0 5 +10633 4 0 +3447 0 2 +4965 4 0 +26277 0 3 +29394 1 0 +32032 1 0 +21855 2 0 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