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The Matlab code I wrote to compute the speckle modulation for the publication:

Mike Adams, Alex Matov, Daphne Yarar, Steph Gupton, Gaudy Danuser, Clare Waterman "Signal Analysis of Total Internal Reflection Fluorescent Speckle Microscopy and Wide-Field Epi-Fluorescence FSM of the Actin Cytoskeleton and Focal Adhesions in Living Cells (2004)"

My software provides the number of speckles in a ROI and their mean modulation; getModulation.m is the main function