diff --git a/aiohttp/test_utils.py b/aiohttp/test_utils.py
index 606f9743317..312a4557267 100644
--- a/aiohttp/test_utils.py
+++ b/aiohttp/test_utils.py
@@ -148,6 +148,7 @@ def __init__(self, app, *,
 
     @asyncio.coroutine
     def _make_factory(self, **kwargs):
+        self.app._set_loop(self._loop)
         yield from self.app.startup()
         self.handler = self.app.make_handler(loop=self._loop, **kwargs)
         return self.handler
diff --git a/changes/2060.bugfix b/changes/2060.bugfix
new file mode 100644
index 00000000000..4c86f8dae76
--- /dev/null
+++ b/changes/2060.bugfix
@@ -0,0 +1 @@
+Fix missing app.loop on startup hooks during tests
diff --git a/tests/test_loop.py b/tests/test_loop.py
new file mode 100644
index 00000000000..8437fb539c2
--- /dev/null
+++ b/tests/test_loop.py
@@ -0,0 +1,20 @@
+import asyncio
+from aiohttp import web
+from aiohttp.test_utils import AioHTTPTestCase, unittest_run_loop
+
+
+class TestCase(AioHTTPTestCase):
+    @asyncio.coroutine
+    def get_application(self):
+        app = web.Application()
+        app.on_startup.append(self.on_startup_hook)
+        return app
+
+    @asyncio.coroutine
+    def on_startup_hook(self, app):
+        self.startup_loop = app.loop
+
+    @unittest_run_loop
+    @asyncio.coroutine
+    def test_on_startup_hook(self):
+        assert self.startup_loop is not None