diff --git a/airflow/providers/amazon/aws/operators/glue.py b/airflow/providers/amazon/aws/operators/glue.py
index 05eafe72692f7..0602e1d5acd23 100644
--- a/airflow/providers/amazon/aws/operators/glue.py
+++ b/airflow/providers/amazon/aws/operators/glue.py
@@ -104,7 +104,9 @@ def execute(self, context: 'Context'):
 
         :return: the id of the current glue job.
         """
-        if not self.script_location.startswith(self.s3_protocol):
+        if self.script_location is None:
+            s3_script_location = None
+        elif self.script_location.startswith(self.s3_protocol):
             s3_hook = S3Hook(aws_conn_id=self.aws_conn_id)
             script_name = os.path.basename(self.script_location)
             s3_hook.load_file(