KD-MISC_single-cell-meta-analysis

Analysis scripts for the manuscript "Single-cell Meta-analysis Of Neutrophil Activation in Kawasaki Disease and Multi-system Inflammatory Syndrome in Children" Disease Molecular Mechanism Investigation Lead Target Discovery Therapeutic Drug Identification

Folder Structure

1. Processing, Integration, Annotation, Re-Clustering

Analysis and plotting scripts used for pre-processing all scRNA-seq data until re-clustering

2. Differential Expression & SNEP Identification

Analysis and plotting scripts for differential expression analysis and SNEP identification

3. Single-cell hdWGCNA

Analysis and plotting scripts for single-cell high-dimensional weighted gene co-expression network analysis

4. Target-Pathway Correlation Analysis

Analysis and plotting scripts for correlation analyses

5. Differential Abundance Testing

Analysis and plotting scripts for testing the differential abundance of cell types and neutrophil subpopulations

6. Validation - MIS-C bulk RNA-seq

Analysis and plotting scripts for the validation study (bulk RNA-seq) for independent MIS-C data

7. Validation - KD scRNA-seq

Analysis and plotting scripts for the validation study (single-cell RNA-seq) for independent KD data

8. Visualization Plots

Analysis and plotting scripts for other statistical testing and visualizations including violin, pie chart, bar, box, scatter plots