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Merge pull request #103 from AlexsLemonade/allyhawkins/missing-spaces
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Add some missing spaces before refs
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allyhawkins authored Mar 25, 2024
2 parents 397aa4e + 06d50a3 commit 8558b4f
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Expand Up @@ -60,7 +60,7 @@ This unfiltered counts matrix is stored in a `SingleCellExperiment` object [@doi
The unfiltered gene by cell counts matrices are filtered to remove any barcodes that are not likely to contain cells using `DropletUtils::emptyDropsCellRanger()`[@doi:10.1186/s13059-019-1662-y], and all cells that pass are saved in a `SingleCellExperiment` object and a file with the suffix `_filtered.rds`.
Low-quality cells are identified and removed with `miQC` [@doi:10.1371/journal.pcbi.1009290], which jointly models the proportion of mitochondrial reads and detected genes per cell and calculates a probability that each cell is compromised.
The remaining cells' counts are normalized [@doi:10.1186/s13059-016-0947-7], and reduced-dimension representations are calculated using both principal component analysis (PCA) and uniform manifold approximation and projection (UMAP) [@arxiv:1802.03426].
Finally, cell types are classified using two automated methods, `SingleR`[@doi:10.1038/s41590-018-0276-y] and `CellAssign`[@doi:10.1038/s41592-019-0529-1].
Finally, cell types are classified using two automated methods, `SingleR` [@doi:10.1038/s41590-018-0276-y] and `CellAssign` [@doi:10.1038/s41592-019-0529-1].
The results from this analysis are stored in a processed `SingleCellExperiment` object saved to a file with the suffix `_processed.rds`.

To make downloading from the Portal convenient for R and Python users, downloads are available as either `SingleCellExperiment` or `AnnData`[@doi:10.1101/2021.12.16.473007] objects.
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